Plan PCR experiment
pcr_plan.RdPlan PCR experiment
Usage
pcr_plan(
data,
n_primers,
format = 384,
exclude_border = TRUE,
primer_names = NULL,
headless = TRUE,
has_names = TRUE
)Arguments
- data
a data.frame, with samples as the first column (if
has_names = TRUE) and RNA concentrations as the second (or first, ifhas_names = FALSE)- n_primers
integer. Number of primers to be used in the experiment.
- format
integer. 96 or 384 - the number of wells of the plate planned to be used
- exclude_border
logical. Should the border be excluded to avoid edge effects? Default is TRUE.
- primer_names
character vector. Names of primers.
- headless
logical. If FALSE, return invisible and redirect to shiny application.
- has_names
logical. Is the first column the names of the samples?
Examples
dummy_rna_conc |>
pcr_plan(n_primers = 3)
#> $mm_prep
#> # A tibble: 4 × 2
#> reagent vol
#> <chr> <dbl>
#> 1 2X RT-PCR Buffer 206.
#> 2 Primer 20.6
#> 3 25X RT-PCR Enzyme 16.5
#> 4 Nuclease Free H2O 103.
#>
#> $sample_prep
#> # A tibble: 8 × 7
#> sample conc dilution_factor diluted_concentration diluted_rna_to_add
#> <chr> <dbl> <int> <dbl> <dbl>
#> 1 24hr_DMSO_1 127. 1 127. 1.18
#> 2 48hr_DMSO_1 93 1 93 1.61
#> 3 24hr_1uM_1 143. 1 143. 1.05
#> 4 48hr_1uM_1 67.1 1 67.1 2.24
#> 5 24hr_DMSO_2 88.3 1 88.3 1.70
#> 6 48hr_DMSO_2 124. 1 124. 1.21
#> 7 24hr_1uM_2 94.2 1 94.2 1.59
#> 8 48hr_1uM_2 80.2 1 80.2 1.87
#> # ℹ 2 more variables: water_to_add <dbl>, final_vol <dbl>
#>
#> $plate
#>
#> 3
#> ______
#> 1 | ◯ ◯ ◯
#>
#>
#> Start corner: tl
#> Plate dimensions: 16 x 24
#>
#> $n_primers
#> [1] 3
#>
#> $format
#> [1] "384"
#>
#> $exclude_border
#> [1] TRUE
#>
#> $primer_names
#> NULL
#>