Read and tidy a Synergy2 file

Usage

read_synergy2(path)

Arguments

path: Path to the Synergy2 .txt file

Value

A list, with the following form:

data: a tibble with all well-level experimental data combined
wavelengths: a numeric vector containing the wavelengths read
metadata: A list containing residual, non-well data

Examples

system.file("extdata", "bca-assay-all-export.txt", package = "mop") |>
  read_synergy2()
#> $data
#> # A tibble: 96 × 8
#>     .row  .col WellID `Conc/Dil` Name  nm562 Blank562 Conc  
#>    <int> <dbl> <chr>       <dbl> <lgl> <dbl>    <dbl> <chr> 
#>  1     1     1 BLK            NA NA    0.091    0.001 <0.000
#>  2     1     2 STD1            0 NA    0.092    0.002 <0.000
#>  3     1     3 STD2           10 NA    0.142    0.051 10.704
#>  4     1     4 STD3           25 NA    0.198    0.108 26.815
#>  5     1     5 STD4           50 NA    0.288    0.197 52.708
#>  6     1     6 STD5          100 NA    0.438    0.348 95.863
#>  7     1     7 SPL1           NA NA    0.096    0.006 <0.000
#>  8     1     8 SPL2           NA NA    0.112    0.022 2.073 
#>  9     1     9 SPL3           NA NA    0.387    0.296 81.190
#> 10     1    10 SPL4           NA NA    0.222    0.132 33.720
#> # ℹ 86 more rows
#> 
#> $wavelengths
#> [1] 562
#> 
#> $metadata
#> $metadata$`Procedure Summary`
#>         name         value
#> 1 Plate Type 96 WELL PLATE
#> 2       Read     Read: 562
#> 
#> $metadata$`Data Reduction Summary`
#>             name     value
#> 1 Transformation Blank 562
#> 2 Curve Analysis     Curve
#> 3 Curve Analysis      Conc
#> 
#> $metadata$`Curve Fitting Results`
#>   CurveName CurveFormula       A      B    R�
#> 2     Curve      Y=A*X+B 0.00348 0.0143 0.993
#> 
#> $metadata$`Curve Fitting Details`
#> # A tibble: 2 × 7
#>   CurveName CurveFormula Parameter   Value Std.Error `95%CImin` `95%CImax`
#>   <chr>     <chr>        <chr>       <dbl>     <dbl>      <dbl>      <dbl>
#> 1 Curve     Y=A*X+B      A         0.00348  0.000173    0.00293    0.00402
#> 2 NA        NA           B         0.0143   0.00888    -0.0139     0.0425 
#> 
#> $metadata$`Data Audit Trail`
#> # A tibble: 3 × 4
#>   Date                   User          Event                             Comment
#>   <chr>                  <chr>         <chr>                             <lgl>  
#> 1 12/15/2005 11:31:29 AM Administrator "Protocol \"S:\\TempDEC\\Lenore\… NA     
#> 2 2/8/2006 9:41:52 AM    User          "Plate read from file \"C:\\KC4 … NA     
#> 3 2/8/2006 9:41:52 AM    User          "Plate read successfully complet… NA     
#> 
#> 
#> attr(,"class")
#> [1] "synergy2"