Calculate normalized ODs without fitting

It can be useful to calculate normalized ODs without fitting, particularly if you would like to use geom_mtt, or need to manipulate your data downstream.

Usage

mtt_calc(
  data,
  signal = "nm562",
  background = "nm660",
  dose = "dose",
  out = "div",
  .by = NULL
)

Arguments

data

A data.frame of absorbances, containing at least the signal, background, and dose columns (see below)

signal

The name of the column that contains the signal absorbances

background

The name of the column that contains the background absorbances. If NULL, will just use signal.

dose

The name of the column containing the concentrations of the drug. Will use the lowest concentration as the baseline.

out

The name of the output column

.by

Character vector of columns to summarize by. Other columns will be dropped